Revvity ViaStain No-Wash Annexin V-FITC Kit for Celigo, 5 x 96-well plates

Annexin V is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. In healthy cells, PS is normally only found on the intracellular leaflet of the plasma membrane, but during apoptosis, PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify the PS on the surface of apoptotic cells. By simultaneously staining the cells with Annexin V, propidium iodide (PI) and Hoechst we can discriminate between different populations of cells. This kit is for use with the Celigo Image Cytometry Instrument.