Revvity Phospho-SMAD2 (Ser465/467) LANCE Ultra TR-FRET Cellular Detection Kit, 500 Assay Points

Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4012S.

Formats:

• Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
• Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE^® and LANCE^® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

SMAD proteins are transcriptional activators activated by bone morphogenic proteins (BMPs). These ligands induce dimerization of SMAD proteins, whereby they translocate to the nucleus and bind target genes. SMAD1, 2, and 3 constitute receptor-regulated SMADs.