Revvity PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol

PhenoVue™ Fluor 647 live cell actin stain is a no wash, cell permeable fluorogenic dye which specifically bind to actin filaments (F-actin) in live cells. Sensitive, rapid and photostable, PhenoVue Fluor 647 live cell actin stain exhibits far-red emission and can be multiplexed with blue, green and orange colors such as PhenoVue nuclear, lysosomal, mitochondrial or tubulin stains. 

Like other actin stains derived from jasplakinolide, cytotoxicity can be observed with long exposure time (>24h) which can be significantly limited at concentrations comprised between 30 and 300nM while maintaining high brightness and image quality.

Depending on the cellular model, intracellular retention of PhenoVue Fluor 647 live cell actin stain can be further improved in the presence of efflux pump inhibitor such as PhenoVue Probenecid, Ready to Use Solution.

Features:

• Numbers of Vials Per Unit : 1
• Quantity or Volume Per Vial: 5 nmoles
• Form: Liquid (DMSO)
• Storage: -16 °C
• Recommended Working Concentration: 100 nM
• Maximum Excitation Wavelength: 650 nm
• Maximum Emission Wavelength: 670 nm
• Common Filter Set: Cy5
• Live Cell Staining: Yes
• Fixed Cell Staining: Yes
• Equivalent Number of Microplates:
  • 1 to 5 x 96-well microplates
  • 1 to 5 x 384-well microplates
  • 2 to 8 x 1536-well microplates