Revvity PhenoVue Fluor 400LS - Phalloidin

Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.

Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.

PhenoVue Fluor 400LS - Phalloidin can be used to visualize actin filaments in indirect immunofluorescence, immunohistochemistry as well as high-content analysis and screening applications. ​​Its unique photophysical property enables extended multiplexing capability by adding a fifth color while ensuring no spectral overlap, high brightness, as well as photostability.

Features

• Number of Vials Per Unit: 1
• Quantity or Volume Per Vial: 300 units (10 nmoles; 12 µg)
• Form: Desiccated
• Storage: -20 °C
• Recommended Working Concentration: 165 nM (0.20 µg/mL)
• Maximum Excitation Wavelength: 395 nm
• Maximum Emission Wavelength: 585 nm
• Live Cell Staining: No
• Fixed Cell Staining: Yes
• Equivalent Number of Microplates:
  • 2 - 6 x 96-well microplates
  • 2 - 6 x 384-well microplates
  • 3 - 10 x 1536-well microplates