Revvity NEXTFLEX RiboNaut rRNA Depletion Kit (Human / Mouse / Rat)

• Hybridization and bead-based protocol for rRNA depletion
• Optimized for use with 5 ng – 1 µg of total RNA as starting material
• Tested using the NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 and automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
• Versions are available for automated and manual rRNA depletion

The NEXTFLEX RiboNaut™ Kit Ensures Effective rRNA Depletion using Hybridization Technology

The NEXTFLEX RiboNaut rRNA depletion kit (human / mouse / rat) utilizes subtractive hybridization technology consisting of a mixture of biotinylated oligos complementary to rRNAs to pull them out of the sample using streptavidin-coated magnetic beads. The kit has been optimized for use with 5 ng – 1 µg of total RNA as starting material to deplete cytoplasmic and mitochondrial rRNAs. It has been verified to be compatible with human, mouse, and rat RNA and it may be compatible with other mammalian species.