Revvity HTRF Human Total Tau Detection Kit, 50,000 Assay Points

The Total Tau cell-based assay is designed to monitor the expression level of Tau proteins, both phosphorylated and unphosphorylated. It uses the same buffers as our Phospho-Tau kits, and enables the analysis of phosphorylated and total proteins from a single sample.

TAU (Tubulin Associated Units), also called MAPT (Microtubule Associated Protein Tau), is a phosphoprotein largely expressed in neurons of the Central Nervous System and involved in microtubule assembly and stability.

Tau contains around of 85 potential phosphosites that can be largely phosphorylated by many kinases such as CDK5, MAP kinase, or GSK3β, the main kinase of Tau. Tau hyperphosphorylation induces a microtubule disruption, Tau aggregation into paired helical filaments or neurofibrillary tangles, and cell death.

Tau hyperphosphorylation and aggregation are involved in numerous neurodegenerative diseases such as Tauopathy, and Parkinsons and Alzheimers Diseases. These two phenomena associated with amyloid-β aggregation are major hallmarks of Alzheimers Disease.