Revvity HTRF Human Total SYK Detection Kit, 50,000 Assay Points

The Total SYK cellular assay monitors total SYK, and can be used as a normalization assay with our phospho-SYK (Tyr525/526) kit. This kit is compatible with the buffers from the phospho-SYK kit, SO the same lysate can be used for analyses of both the phosphorylated and the total protein populations.

SYK (Spleen Tyrosine Kinase) is a cytoplasmic tyrosine/serine kinase which plays a crucial role in signal transduction, especially in immune cells such as B cells, macrophages, microglia (brain-resident macrophages), neutrophils and mast cells. Upon cell stimulation, SYK interacts with the cytoplasmic domains of activated immune receptors and binds to the specific phosphorylated receptor motifs (called "p-ITAMs"), leading to its activation by auto-phosphorylation of its catalytic domain at Tyr525/526.

SYK phosphorylation is a readout of key inflammatory pathways such as BCR, FcR and TREM2 signaling pathways that are altered in various pathologies such as auto-immune diseases, neurodegeneration, cancers and allergic disorders.