Revvity HTRF Human Total Estrogen Receptor α Detection Kit, 50,000 Assay Points

The HTRF Human Estrogen Receptor Alpha detection assay monitors the expression of endogenous or overexpressed Estrogen Receptor Alpha in various cells.

Estrogen Receptor Alpha is a type of nuclear receptor that is activated by estrogen hormones, like Estradiol (E2).

Estrogens bind to the ligand binding domain of the Estrogen Receptor Alpha, resulting in receptor homodimerization and translocation to the nucleus. This activates downstream gene expression and the activation of signaling pathways, like the PI3K/AKT pathway. The gene expression induced by Estrogen Receptor Alpha results in cell division and proliferation. A dysregulation of the Estrogen Receptor Alpha pathway could lead to oncogenic phenotypes, and Estrogen Receptor Alpha activation or overexpression has been widely linked to breast cancer (in more than 70% of the cases). Estrogen Receptor Alpha has therefore become an important drug target for the pharmaceutical industry. The development of PROTAC compounds to target Estrogen Receptor Alpha degradation is a part of the therapeutic strategy to target Estrogen Receptor Alpha positive cancers. Alternatively Selective Estrogen Receptor Degrader (SERD) can be detected with this kit, but not Selective Estrogen Receptor Modulator (SERM) as they only impact downstream signaling and not the expression level of the protein.