Revvity HTRF Human Phospho-Cyclin D1 (Thr286) Detection Kit, 50,000 Assay Points

This HTRF cell-based assay conveniently and accurately detects phosphorylated Cyclin D1 at Thr286. ​​​​​​​

Cyclin D family members (including Cyclin D1, Cyclin D2, and Cyclin D3) are important regulators of cell cycle progression. They are integral mediators of growth factor-dependent G1 to S phase progression by playing the role of allosteric regulators of CDK4/6.

Cyclin D1 is upregulated in presence of extracellular mitogenic stimuli, and is then rapidly downregulated by proteasomal degradation following its phosphorylation on Thr286 by the kinase GSK-3β.

Cyclin D1 is an oncogene whose overexpression results in dysregulated CDK4/6 activity and leads to tumorigenesis. Cyclin D1 overexpression may be caused by gene amplification or chromosomal rearrangements, or as a result of impaired degradation of the protein. Mutations and deletions affecting its phosphorylation on Thr286 have been reported in several human cancers.