Revvity HTRF Human IFN-β Detection Kit, 100,000 Assay Points

Type I interferons, interferon alpha (IFN-α) and interferon beta (IFN-β), play essential roles in innate immune response to viral infections. IFN-β is produced by fibroblasts, macrophages, and dendritic cells, as well as endolial cells. expression of IFN-β gene is controlled by transcription factors like IRF-3, c-Jun/ATF-2, and NF-kappaB downstream of Pattern Recognition Receptors, such as Toll-like receptors. In particular, IFN-β is primary functional readout of cGas-cGAMP-STING pathway activation. Once secreted, IFN-β binds and activates IFNAR1/IFNAR2 receptor complex, which relies on STAT1/2 transcription factors. Phosphorylated STAT1/2 leads to induction of anti-viral gene expression.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.