Revvity HTRF Human & Mouse Phospho-p62/SQSTM1 (Ser403) Detection Kit, 50,000 Assay Points

This HTRF cell-based assay conveniently and accurately quantifies phosphorylated p62/SQSTM1 at Ser403. p62/SQSTM1 is a ubiquitin-binding protein Involved in the regulation of autophagy. This is a cellular mechanism which enables the clearance of abnormal proteins or organelles, and it plays a role in cancer, neurodegenerative diseases, as well as cardiovascular and infectious diseases.

p62/SQSTM1 functions as a bridge between polyubiquitinated cargo protein and autophagosomes by interacting directly with both the cargo protein and a protein called LC3, which is involved in autophagosome formation. Recently, p62/SQSTM1 activation has been associated with ULK1 and mTor- induced phosphorylation on different residues, like Ser 403.

The activated autophagy pathway promotes the degradation of p62-associated ubiquitinated protein cargoes.