Revvity HTRF Human & Mouse Total CDK2 Detection Kit, 50,000 Assay Points

The Total CDK2 cellular assay monitors total CDK2, and can be used as a normalization assay with our phospho-CDK2 (Tyr15) kit. This kit is compatible with the buffers from the phospho-CDK2 kit, SO the same lysate can be used for analyses of both the phosphorylated and the total protein populations.

CDK2 (Cyclin-Dependent Kinase 2) is a member of the subfamily of CDKs that coordinate cell cycle progression in mammalian cells (including also CDK1, CDK4, and CDK6). CDK2 is activated by interaction with Cyclin E and Cyclin A during the late G1 phase and the S phase respectively, and is also regulated by two major phospho-sites. Phosphorylation at Tyr15 by the kinase Wee1 is inhibitory by preventing ATP binding, whereas Tyr15 dephosphorylation by the phosphatase Cdc25A and phosphorylation at Thr160 by CAK (CDK Activating Kinase) is required for its full activity.

CDK2 inhibitory phosphorylation at Tyr15 is essential for maintaining genome integrity and preventing DNA damage during the S phase. The Wee1/Cdc25A axis is therefore an attractive target for cancer therapy and may represent a unique approach to sensitize cancer cells with hyperactive CDK2.