Revvity HTRF Human & Mouse Phospho-FAK (Tyr397) Detection Kit, 50,000 Assay Points

This HTRF cell-based assay conveniently and accurately quantifies phosphorylated FAK at Tyr397 (residue for human, rat, and chicken, equivalent to Y428 for mouse). FAK, or Focal Adhesion Kinase, is a cytoplasmic tyrosine kinase controlling cell adhesion, migration, proliferation, and survival. FAK is a central regulator of ECM/integrin and cadherin signaling, and plays a role in cell adhesion, cell junction, migration, survival, and mechanosensing. FAK can be activated by Growth Factor Receptors, Cytokine Receptors, and GPCRs. Increased FAK expression and phosphorylation is associated with increased tumor cell adhesion, migration, invasion in several cancers, and with fibroblast migration on fibronectin in tissue fibrosis.