Revvity AlphaLISA Epigenetics Buffer 1, 5X (10 mL)

Several pre-formulated buffers are available for Alpha assays. These have have been developed to meet the varying needs of different Alpha assays, and one of them may be a good choice for an assay that you are developing. This buffer prevents the non-specific binding of the highly-charged histone H3 (1-21) peptide to Donor and Acceptor beads in AlphaLISA epigenetic assays. Validated with Acceptor beads anti-H3K9ac (AL114), anti-H3K4me1-2 (AL116) and anti-H3K9me2 (AL117). It is used at the detection step for the dilution of Acceptor and Donor beads, not for the enzymatic reaction step. This buffer is not recommended for assays using full length histone proteins or nucleosomes.

Formulation: proprietary