Revvity Alpha TE Lysis Buffer Selection Kit

This kit contains 10 mL each of buffers 1, 2, 3, 4, and 5 for use in cell-based target engagement assays.

AlphaLISA TE Cell Lysis Buffer 1: This formulation is the most aggressive detergent formulation, containing divalent cation chelators, low salt concentration, moderate glycerol concentration.

AlphaLISA TE Cell Lysis Buffer 2: Less aggressive detergent formulation, optimized for lysis of a broad range of cells without releasing nuclear DNA and minimally disrupting protein interactions; physiological salt concentrations.

AlphaLISA TE Cell Lysis Buffer 3: Medium detergent concentration, strongest pH buffering capacity, no glycerol, low salt, 0.1% casein

AlphaLISA TE Cell Lysis Buffer 4: Medium-High detergent concentration, contains divalent cation chelators, close to physiological osmotic strength, no glycerol

AlphaLISA TE Cell Lysis Buffer 5: Medium-High detergent concentration, contains divalent cation chelator, close to physiological osmotic strength, highest glycerol concentration