Electron Microscopy Sciences Propane Transfer System

Electron Microscopy Sciences Cryo workstation allows for a reliable virtually routine freezing of biological samples. The ems-002 achieves ultra-rapid cooling by quenching the sample in liquid propane or other suitable cryogen, cooled to near liquid nitrogen temperature. This is accomplishes by using a stainless steel vacuum insulated dewar and copper condensation chamber. Liquid nitrogen is poured into the dewar. The initial cooling of the cryogen condensation chamber takes about 15 to 20 minutes. Gaseous propane is then admitted to the condensation chamber slowly enough to allow it to condense and fill the chamber with liquid propane. The propane is ledtto cool to near liquid nitrogen temperature supervised by the digital temperature controller while the sample is being prepared for freezing. The reate of cooling can be increase or decrease by adjusting the level of liquid nitrogen in the dewar. - EMS