Electron Microscopy Sciences Trypsin Kit Large

Electron Microscopy Sciences Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (ffpe) tissue sections prior to application of antibodies. In ihc most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in ihc. Trypsin digestion improves immunoreactivity of some antigens in ffpe tissue sections. For cytokeratin clone ae3 and ae1/ae3, this enzyme works much better than boiling the tissue with citrate buffer. Intended use: use as antigen retriever for some antibodies in ihc. Please refer to primary antibody protocol for ihc. Reagent: this enzyme is supplied as concentrated form along with buffer. - EMS (Additional S&H or Hazmat Fees May Apply)