Takara NucleoSnap Plasmid Midi, 10 Preps

Endotoxins are copurified during plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Macherey-Nagel has developed a new column format and a novel buffer chemistry to enable vacuum-processed isolation of transfection-grade plasmid DNA in a midiprep format.

Higher, more consistent yields with NucleoSnap Plasmid Midi.Multiple plasmid preps were performed in parallel from the indicated volumes of bacterial cultures using NucleoSnap Plasmid Midi, the Plasmid Plus Midi Kit from Qiagen (Q), and the ZymoPURE II Plasmid Midiprep Kit from Zymo (Z), respectively. Quantification of purified plasmid from each prep indicated that NucleoSnap Plasmid Midi provided higher yields than the Qiagen kit and more consistent results than the Zymo kit.