Takara Buffer NTB, 150 mL

For clean-up of SDS-containing samples when using NucleoSpin Gel and PCR kits. Includes binding Buffer NTB, 1 bottle of 150 mL.

Required Products

NucleoSpin Gel and PCR Clean-up (250) 250 preps for PCR-clean-up and gel extraction - NucleoSpin and PCR Clean-up Columns, Collection Tubes, buffers

The NucleoSpin Gel and PCR cleanup kit procedure is the easiest way to purify DNA fragments from agarose gels, and for direct purification of PCR products.The kit includes one buffer for both applications. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin Gel and PCR cleanup kit columns provide convenient performance for PCR cleanup: After addition of Binding Buffer NT the mixture is applied onto the silica membrane. Contaminants are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to NucleoSpin Gel and PCR cleanup kit column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mm Tris/HCl, pH 8.5).