Takara NucleoBond RNA Soil, 20 Preps

The NucleoBond RNA Soil kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate high sample inputs in order to yield 1–10 µg of RNA. Sample lysis using MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineralic and organic soil samples. NucleoBond RNA Soil enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 20 samples.

NucleoBond RNA Soil procedure.

High RNA yields with NucleoBond RNA Soil.Various soil samples (clay, shore soil, and forest soil) were purified in duplicate with NucleoBond RNA Soil using the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding kit protocol. Each RNA sample was eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil provided a higher yield compared to the competitor kit.

Amplifiable RNA for perfect results with NucleoBond RNA Soil.Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil and competitor kit "Q" by following the standard protocol for each kit. RT-PCR was then performed using 40 µl of eluate from each sample (amplicon size = 466 bp). Samples prepared using NucleoBond RNA Soil demonstrated lower Cp values relative to corresponding samples prepared using kit "Q".

NucleoBond RNA Soil enables efficient parallel DNA isolation.Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil in combination with the DNA Set for NucleoBond RNA Soil following the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding protocol. DNA samples were eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil combined with the DNA Set for NucleoBond RNA Soil provided a higher yield of DNA compared to the competitor kit.