Takara NucleoSpin RNA, 50 Preps

The NucleoSpin RNA kit (50 preps) allows for purification for up to 70 μg of total RNA from cultured cells, tissue, bacteria, yeast, and cell-free biological fluids without the use of harsh organic compounds. The kit includes NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, and RNase-free rDNase.

Residual Genomic DNA Comparison on an Agarose Gel.10 μl of each eluate was treated with RNase A and subsequently loaded onto a 1% TAE agarose gel to reveal any contaminating genomic DNA.The NucleoSpin RNA II kit contains DNase I for on-column digestion. For Competitor Q’s kit, an additional on-column DNA digestion set was purchased separately in order to perform the digest. Lane m: λ/HindIII DNA size marker. Note: Optimized DNase I treatment has eliminated genomic DNA in total RNA samples purified with the NucleoSpin RNA II kit. Even after DNA digestion, Competitor Q’s kit showed evidence of genomic DNA contamination.