Takara NucleoSpin Gel & PCR Clean-up Midi, 20 Preps

The NucleoSpin Gel & PCR Clean-up Midi kit includes spin columns, collection tubes, and buffers for purification of DNA from PCR reactions or agarose gels. The kit enables recovery of DNA fragments ranging in size from 50 bp–20 kb, and can be used to process up to 4 mL of PCR reaction mixture or 4 g of agarose gel in one prep. The binding capacity of the midi spin column is 75 µg, and typical elution volumes range from 200–400 µl. The kit employs a user-friendly workflow that enables processing of 6 samples in ~25 minutes.

Cat. # 740986.20 includes sufficient quantities of reagents and materials for performing 20 preps.

Complete primer removal down to 65 bp using NucleoSpin Gel and PCR Clean-up Midi.PCR products of 252, 164, 100, and 65 bp were spiked with primers and purified with NucleoSpin Gel and PCR Clean-up Midi. Elution was performed using 400 µl of elution buffer. Shown in the image are the samples prior to (Lane 1) and after (Lane 2) PCR purification on a 2% TAE agarose gel.

Gel extraction using NucleoSpin Gel and PCR Clean-up Midi.pGEM T Easy vectors containing two different inserts were digested using EcoRI and analyzed by electrophoresis. Digested inserts and vectors were cut out of the gel and purified with NucleoSpin Gel and PCR Clean-up Midi. Gel purification was analyzed on a 1% TAE agarose gel. Samples in each lane are as follows:

1. pGEM T Easy with 645-bp insert prior to digestion2. pGEM T Easy vector backbone and 645-bp insert after digestion3.645-bp insert after gel extraction4. pGEM T Easy vector backbone after gel extraction5. pGEM T Easy with 982-bp insert prior to digestion6. pGEM T Easy vector backbone and 982-bp insert after digestion7.982-bp insert after gel extraction8. pGEM T Easy vector backbone after gel extraction