Takara NucleoSpin VET, 250 Preps

NucleoSpin VET is designed for the isolation of viral DNA/RNA and bacterial DNA from veterinary samples such as serum or plasma, whole blood, and homogenized tissue suspensions, etc. The kit includes columns, collection tubes (2 mL), collection tubes for lysis and elution (1.5 mL), buffers, liquid Proteinase K, and carrier RNA (lyophilized).

Reliable viral DNA and RNA recovery from bovine blood with NucleoSpin VET.The NucleoSpin VET kit was used for viral DNA and RNA isolation from bovine blood. Thawed bovine blood was spiked with lumpy skin disease virus (LSDV), bovine coronavirus (BoCoV), and foot-and-mouth disease virus (FMDV) at various dilution levels. Virus-spiked blood samples were purified using NucleoSpin VET and eluates were analyzed via RT-qPCR using AgPath-IDTM One-Step RT-PCR reagents (ThermoFisher Scientific). The NucleoSpin VET kit demonstrated consistent and reliable viral RNA and DNA recovery from high to low viral titer samples (FMDV titer in 1:10,000 dilution was below detection limit). Data kindly provided by Hanna Keck and Michael Eschbaumer, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health of Germany.

Comparison of manual and automated extraction methods in detection of foot-and-mouth disease virus in bovine milk and skin lesions.Nucleic acids were isolated from foot-and-mouth disease virus (FMDV)-positive bovine milk and skin lesions using NucleoSpin VET (manual single spin purification) or NucleoMag VET (magnetic bead-based automated extraction). For NucleoMag VET, a 100 μL sample input was used. Eluates were subsequently analyzed via RT-qPCR using AgPath-IDTM One-Step RT-PCR reagents (ThermoFisher Scientific). Ct values for each sample extracted using NucleoSpin VET were comparable to samples extracted using NucleoMag VET. Data kindly provided by Hanna Keck and Michael Eschbaumer, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health of Germany.

Market-leading performance compared to competitor products.Extractions from cloacal swab solutions, chicken liver tissue, bovine blood, and bovine plasma samples were spiked with MS2 viral RNA. Nucleic acid purifications were performed using NucleoSpin VET or using an extraction kit from Competitor I. Following nucleic acid extraction, RT-qPCR analysis was performed using the Bioline SensiFASTTM SYBR Lo-ROX One-Step kit to determine the viral titer load in the different sample matrices. Extraction with NucleoSpin VET resulted in higher viral RNA recovery compared to Competitor Is kit.

Standard NucleoSpin VET nucleic acid purification protocol workflow.

Comparison of NucleoSpin VET standard and FastTrack protocols.Canine distemper virus (CDV) (Panel A) or porcine enterovirus (PEV) (Panel B) were spiked into bovine plasma or saliva samples. Then, nucleic acids were isolated from spiked samples using the standard or FastTrack NucleoSpin VET protocols and recovery of CDV and PEV were assessed via RT-qPCR. Ct values were comparable for the standard and Fasttrack protocols.

Comparison of manual and automated extraction methods for high to low viral titer samples.Varying dilutions of MS2 viral RNA were spiked into bovine plasma to simulate low, medium, and high viral titer samples. Nucleic acid purifications were performed using NucleoSpin VET (manual single-spin purification) or NucleoMag VET (magnetic bead-based automated extraction) and analyzed via RT-qPCR using the Bioline SensiFASTTM SYBR Lo-ROX One-Step kit. Ct values for low, medium, and high viral titer samples extracted using NucleoSpin VET were comparable to those samples extracted using NucleoMag VET.