Takara NucleoSpin Inhibitor Removal, 50 Preps

The NucleoSpin Inhibitor Removal kit is designed for fast and efficient cleanup of pre-purified DNA samples containing substances such as humic acids, heme, polyphenols, or tannins that inhibit PCR amplification. The presence of such inhibitors may be due to insufficient purification procedures or processing of challenging sample types such as soil, blood, fruit, processed food, etc. The kit employs silica membrane technology and a simple mini-spin protocol that enables processing of 6 samples in 15 minutes, with typical recoveries >75%.

Cat. # 740408.50 includes reagents and materials for processing 50 samples.

Higher purity with NucleoSpin Inhibitor Removal.Contaminated DNA samples were processed with NucleoSpin Inhibitor Removal (MN) and two competitor kits (Z and Q). For each sample type, cleanup with NucleoSpin Inhibitor Removal was associated with higher A260/280and A260/230ratios as compared to the competitors.

Superior DNA recovery with NucleoSpin Inhibitor Removal.Contaminated DNA samples were processed with NucleoSpin Inhibitor Removal (MN) and two competitor kits (Z and Q). As shown, DNA recovery was higher with NucleoSpin Inhibitor Removal as compared to the competitors.

Efficient DNA amplification following cleanup with NucleoSpin Inhibitor Removal.Contaminated DNA samples were processed with NucleoSpin Inhibitor Removal (MN) and two competitor kits (Z and Q), and then subjected to PCR amplification followed by agarose gel electrophoresis. In contrast with untreated samples, and those processed with the competitor kits which failed to yield PCR products, samples processed using NucleoSpin Inhibitor Removal could be reliably amplified.