Takara NucleoSpin RNA Plus XS, 10 Preps

The NucleoSpin RNA Plus XS kit (10 preps) is optimized for efficient isolation of high-quality RNA from extra-small (XS) amounts of cells and tissue. The included NucleoSpin gDNA Removal Column XS provides enzyme-free DNA removal, saving 15 min in prep time compared to standard DNA digestion methods. NucleoSpin RNA Plus XS uses a novel lysis buffer chemistry that eliminates the need for reducing agents such as beta-mercaptoethanol or TCEP, avoiding unpleasant odors and health concerns.

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS.Total RNA was purified from the indicated amounts of HeLa cells using NucleoSpin RNA Plus XS or NucleoSpin RNA XS, respectively, by following the standard protocol for each kit (the former employs a gDNA removal column while the latter includes an on-column DNase digestion step). The amount of residual genomic DNA in the purified sample varied depending on the input amount, but for the input range of 10–10,000 cells, the NucleoSpin RNA Plus XS kit provided more efficient removal of genomic DNA than the NucleoSpin RNA XS kit.

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS.Total RNA was purified from the indicated amounts of mouse liver tissue using NucleoSpin RNA Plus XS or kits from Competitor Q/Q+, I, and Z, respectively. Yields of total RNA obtained with NucleoSpin RNA Plus XS were greater than or comparable to yields obtained with each competitor kit for each of the input amounts processed.