Revvity HTRF Human Pro IL-1β Detection Kit, 100,000 Assay Points

Pro IL-1β, also known as IL-1β precursor, is a 31 kDa protein precursor of IL-1β. It is produced in response to molecular motifs carried by pathogens called ‘pathogen associated molecular patterns’ (PAMPs). Induction of pro IL-1β expression is referred to as a priming step and is an inefficient secretion stimulus. The processing and cleavage of pro IL-1β into active IL-1β is conditioned by the activation stage involving PAMP, or DAMP (danger associated molecular pattern, endogenous molecules released from dead cells).

Pro IL-1β is cleaved by the pro-inflammatory protease caspase-1, activated via recruitment to a multi-protein complex termed the inflammasome.

HTRF assays offer many advantages over other technologies:

• Homogeneous add-and-read format
• No wash steps
• Low background
• Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
• Stable signal, providing flexibility in readout time or size of assays