Revvity miND Spike-In Controls

miND Spike-In Controls calibrate and quality check small RNA-seq by adding defined small RNA standards to each sample before library prep. They verify the dynamic range of the assay and enable absolute quantitation, converting reads into copies per microliter for more reliable cross-sample and cross-batch comparisons, especially in biofluids and other low-input matrices.

• Seven synthetic spike-ins supplied in defined ratios to span typical range of endogenous small-RNA abundances.
• Unique 13-nt core with four randomized bases at both ends, designed to minimize ligation bias; 5′-phosphorylated.
• Absolute quantitation via linear regression to output molecules/µL..
• Simple integration: add 1 µL per RNA sample just before NEXTFLEX small RNA-seq library prep.
• Broad compatibility across species and matrices including plasma, serum, urine, CSF, synovial fluid, EVs, cells, and tissues.
• Simple format: 96 reaction lyophilized.
• Built-in analysis support: TAmiRNA provides ready-to-use scripts and a Docker/Snakemake pipeline on GitHub to quantify miRNAs and the spike-ins, then fit a regression to report absolute unit.