Revvity pHSense Boost Reagent, 0.5 mL

pHSense Eu reagents are europium-labeled, pH-sensitive probes designed for the real-time, no-wash detection of antibody internalization, as well as receptor- and membrane protein-mediated internalization in live-cell, plate-based assays.

pHSense Boost is an innovative reagent developed specifically to enhance pHSense Eu internalization assays by addressing the limitations of narrow assay windows. It effectively reduces background signals and amplifies fold-change measurements, improving assay dynamic range.

A key advantage of pHSense Boost is its ability to enhance assay performance without affecting the pharmacological profiles of tested compounds, ensuring the integrity and reliability of screening and characterization data.

The efficacy of pHSense Boost is influenced by cell type, cell density, and incubation time. As such, its benefits are not universal and require empirical validation for each specific assay condition.