Revvity HTRF FLAG Check Kit, 500 Assay Points

The kit enables the rapid detection of Flag-tagged fusion proteins at every stage of protein production and purification. It can be used to verify that the Flag motif of a fusion protein is accessible to the anti-FLAG antibody or to estimate the concentration of a Flag-tagged protein. When calibrated using the appropriate protein, the assay is remarkably accurate and compatible with the SOPs. It can also be used to eliminate false positives.

HTRF assays offer many advantages over other technologies:

• Homogeneous add-and-read format
• No wash steps
• Low background
• Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
• Stable signal, providing flexibility in time of readout or size of assays