Revvity AlphaLISA SureFire Biotin-Free Human Total STAT4 Detection Kit, 50,000 Assay Points

Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that mediates cellular responses to IL-12 and type I interferons, playing essential roles in Th1 cell differentiation and NK cell activation. STAT4 is activated by phosphorylation at Tyr693, leading to dimerization and nuclear translocation. Activated STAT4 induces expression of IFN-γ and T-bet, promoting Th1 responses and cell-mediated immunity against intracellular pathogens. STAT4 contributes to autoimmune disease pathogenesis, with genetic variants associated with increased risk of rheumatoid arthritis and lupus. Modulating STAT4 activity represents a therapeutic opportunity for enhancing immunity or dampening pathological Th1 responses.

The AlphaLISA SureFire Biotin-Free Human Total STAT4 Detection Kit is a sandwich immunoassay for the quantitative detection of total STAT4 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Biotin Free kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies
• Biotin rich samples

AlphaLISA SureFire Biotin Free kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies