Revvity AlphaLISA SureFire Ultra Human Mutant B-RAF (V600E) Detection Kit, 10,000 Assay Points

B-Raf is a serine/threonine protein kinase and member of the RAF family that functions as a critical component of the RAS-RAF-MEK-ERK signaling cascade. B-Raf is activated by RAS-GTP binding, promoting dimerization and kinase activation, leading to phosphorylation of MEK1/2. The V600E mutation in B-Raf, found in approximately 50% of melanomas, results in constitutive kinase activity and oncogenic transformation. Other B-Raf mutations can paradoxically activate wild-type RAF through enhanced dimerization. Targeted B-Raf inhibitors such as vemurafenib and dabrafenib have revolutionized treatment of B-Raf V600E-mutant melanoma.

The AlphaLISA SureFire Ultra Human Total B-Raf V600E is a sandwich immunoassay for the quantitative detection of total B-Raf V600E in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies