Revvity AlphaLISA SureFire Ultra Human Phospho-STAT1 (Tyr701)/phospho-STAT2 (Tyr690) Complex Detection Kit, 10,000 Assay Points

The phospho-STAT1 (Tyr701)/phospho-STAT2 (Tyr690) complex is the activated heterodimeric transcription factor that mediates type I and type III interferon responses critical for antiviral immunity. Upon interferon receptor engagement, JAK1 and TYK2 phosphorylate STAT1 at Tyr701 and STAT2 at Tyr690, promoting heterodimerization through reciprocal SH2 domain interactions. The phosphorylated heterodimer recruits IRF9 to form the ISGF3 complex, which translocates to the nucleus and binds interferon-stimulated response elements (ISREs) to induce expression of antiviral genes including MX1, OAS, PKR, and ISG15. Unlike STAT1 homodimers that bind GAS elements in response to IFN-γ, the STAT1-STAT2 heterodimer drives a broader interferon-stimulated gene program. Deficiencies in either STAT1 or STAT2 cause severe viral susceptibility, while sustained complex activation contributes to autoimmune diseases and interferonopathies. Many viruses encode proteins that degrade or inhibit STAT2 to evade immune responses, highlighting the complex as a critical host defense mechanism.

The AlphaLISA SureFire Ultra Human Phospho-STAT1 (Tyr701)/phospho-STAT2 (Tyr690) Complex Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-STAT1 (Tyr701)/phospho-STAT2 (Tyr690) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies