Revvity AlphaLISA SureFire Ultra Human and Mouse Total HRAS Detection Kit, 10,000 Assay Points

Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) is a small GTPase that functions as a molecular switch in signal transduction pathways controlling cell proliferation, differentiation, and survival. HRAS cycles between inactive GDP-bound and active GTP-bound states, with activation triggered by receptor tyrosine kinases through guanine nucleotide exchange factors (GEFs). Activated HRAS engages multiple effector pathways including RAF/MEK/ERK, PI3K/AKT, and RAL-GDS cascades. Activating mutations in HRAS, particularly at codons 12, 13, and 61, impair GTPase activity resulting in constitutive signaling and oncogenic transformation. HRAS mutations are found in bladder cancer, head and neck squamous cell carcinoma, and Costello syndrome, a developmental disorder with increased cancer risk. Targeting mutant RAS proteins has been challenging, but recent development of KRAS G12C inhibitors has renewed interest in drugging the RAS family.

The AlphaLISA SureFire Ultra Human and Mouse Total HRAS Detection Kit is a sandwich immunoassay for the quantitative detection of total HRAS in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies