Revvity AlphaLISA SureFire Ultra Human and Mouse Total ARID1B Detection Kit, 10,000 Assay Points

AT-Rich Interaction Domain 1B (ARID1B) is a chromatin remodeling protein and core subunit of the SWI/SNF complex that regulates gene expression through ATP-dependent nucleosome repositioning. ARID1B is particularly important for neural development, where it regulates neuronal differentiation, synaptic function, and brain morphogenesis. Haploinsufficiency of ARID1B causes Coffin-Siris syndrome, characterized by intellectual disability and developmental delay. ARID1B mutations are also found in various cancers including neuroblastoma and breast cancer, where loss of function contributes to tumor progression. The tumor suppressor function of ARID1B makes it an important target for developing epigenetic therapies.

The AlphaLISA SureFire Ultra  Human and Mouse Total ARID1B is a sandwich immunoassay for the quantitative detection of total ARID1B in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies