Revvity AlphaLISA SureFire Ultra Human IRAK1 / IRAK4 Complex Detection Kit, 500 Assay Points

The IRAK1/IRAK4 complex is a critical signaling module in TLR and IL-1R pathways that mediates innate immune responses to pathogens. Upon receptor activation, MYD88 recruits IRAK4, which then recruits and phosphorylates IRAK1, forming an active kinase complex. IRAK4 phosphorylates IRAK1 at multiple sites, triggering autophosphorylation and full activation. The activated complex recruits TRAF6, leading to NF-κB and MAPK pathway engagement. Dual IRAK1/IRAK4 inhibitors are being developed as anti-inflammatory therapeutics for autoimmune diseases and MYD88-mutant lymphomas.

The AlphaLISA SureFire Ultra Human IRAK1 / IRAK4 Complex is a sandwich immunoassay for the quantitative detection of IRAK1 / IRAK4 complex in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies