Revvity AlphaLISA SureFire Ultra Human and Mouse Total Src Detection Kit, 500 Assay Points

Src is a non-receptor tyrosine kinase and the prototypical member of the Src family kinases (SFKs) that regulates cell adhesion, migration, proliferation, and survival. Src activity is controlled by intramolecular interactions, with phosphorylation at Tyr527 by Csk promoting an inactive closed conformation, while dephosphorylation and autophosphorylation at Tyr416 activate the kinase. Src integrates signals from receptor tyrosine kinases, integrins, and G-protein coupled receptors to activate downstream pathways including RAS/MAPK, PI3K/AKT, and STAT3. Src is overexpressed or hyperactivated in numerous cancers including colorectal, breast, and lung carcinomas, where it promotes tumor growth, invasion, and metastasis. Src inhibitors such as dasatinib and bosutinib have shown efficacy in CML and are being evaluated in solid tumors, though resistance mechanisms and pathway redundancy have limited their single-agent activity.

The AlphaLISA SureFire Ultra Human and Mouse Total Src Detection Kit is a sandwich immunoassay for the quantitative detection of total Src in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies