Revvity AlphaLISA SureFire Ultra Human and Mouse Phospho-STAT2 Tyr690 Detection Kit, 500 Assay Points

Signal Transducer and Activator of Transcription 2 (STAT2) is a transcription factor that primarily mediates type I and type III interferon (IFN) signaling, playing a central role in establishing antiviral defenses. Upon IFN stimulation, receptor‑associated JAK1 and TYK2 phosphorylate STAT2 (and STAT1). Phosphorylated STAT2 heterodimerizes with STAT1 and associates with IRF9 to form the ISGF3 complex, which binds interferon‑stimulated response elements (ISREs) to induce interferon‑stimulated genes (ISGs), including OAS, PKR (EIF2AK2), and MX1/MX2.

Unlike several other STATs, STAT2 does not bind DNA as a homodimer and exerts most transcriptional functions within ISGF3. In STAT1‑limited contexts, STAT2 can partner with IRF9 to drive a STAT2–IRF9 (ISGF3‑like) response, extending antiviral gene induction.

STAT2 deficiency in humans causes marked susceptibility to viral infections (notably many respiratory RNA viruses, and some DNA viruses), often with severe disease after live‑attenuated vaccines, underscoring STAT2’s non‑redundant role in IFN signaling. Consistently, numerous viruses (e.g., paramyxoviruses, flaviviruses, orthomyxoviruses) encode proteins that degrade, sequester, or block STAT2, highlighting STAT2 as a major target of viral immune evasion.

Because STAT2 tunes IFN pathway amplitude and duration, it represents a potential therapeutic node to enhance antiviral responses or attenuate pathogenic IFN signaling in selected auto‑inflammatory and autoimmune settings.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-STAT2 Tyr690 Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-STAT2 Tyr690 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies