Revvity AlphaLISA SureFire Ultra Human Total SALL4 Detection Kit, 500 Assay Points

Sal-Like Protein 4 (SALL4) is a zinc finger transcription factor that plays crucial roles in embryonic development, stem cell maintenance, and cellular reprogramming. SALL4 regulates pluripotency networks by interacting with core transcription factors including OCT4, SOX2, and NANOG, while also modulating chromatin structure through recruitment of epigenetic regulators. It is essential for early embryogenesis and hematopoietic stem cell function. SALL4 expression is normally restricted to embryonic tissues but becomes aberrantly reactivated in various cancers, including acute myeloid leukemia, hepatocellular carcinoma, and germ cell tumors. In malignancies, SALL4 promotes stemness, drug resistance, and metastasis while inhibiting differentiation and apoptosis. Its oncofetal expression pattern makes SALL4 an attractive target for cancer therapy and a valuable biomarker for diagnosis and prognosis.

The AlphaLISA SureFire Ultra Human Total SALL4 is a sandwich immunoassay for the quantitative detection of total SALL4 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies