Revvity AlphaLISA SureFire Biotin-Free Human and Mouse Total EGR1 Detection Kit, 10,000 Assay Points

Early Growth Response 1 (EGR1) is an immediate-early transcription factor that rapidly responds to diverse stimuli including growth factors, cytokines, and stress signals to regulate cell proliferation, differentiation, and apoptosis. EGR1 is induced within minutes of stimulation through MAPK and calcium signaling pathways, binding to GC-rich sequences in target gene promoters. EGR1 regulates expression of genes involved in cell cycle control (p53, PTEN), differentiation (fibronectin, PDGF), and vascular responses (VEGF, tissue factor). EGR1 functions as both a tumor suppressor and oncogene depending on cellular context, with loss of expression in prostate cancer and glioblastoma but overexpression in breast cancer. EGR1 plays critical roles in vascular injury responses, wound healing, and synaptic plasticity, making it relevant to cardiovascular disease, fibrosis, and neurological disorders.

The AlphaLISA SureFire Biotin-Free Human and Mouse Total EGR1 Detection Kit is a sandwich immunoassay for the quantitative detection of total EGR1 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Biotin Free kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies
• Biotin rich samples

AlphaLISA SureFire Biotin Free kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies