Revvity AlphaLISA SureFire Ultra Mouse Total H2AX Detection Kit, 50,000 Assay Points

H2AX is a histone variant that comprises approximately 10% of total H2A in mammalian cells and serves as a critical sensor and mediator of DNA double-strand break responses. Upon DNA damage, H2AX is rapidly phosphorylated at Ser139 by ATM, ATR, and DNA-PK kinases, forming γH2AX foci that spread megabases from the break site. γH2AX serves as a platform for recruiting DNA repair factors including MDC1, 53BP1, and BRCA1, facilitating homologous recombination and non-homologous end joining. The formation of γH2AX foci is widely used as a biomarker for DNA damage and genotoxic stress in research and clinical settings. Loss of H2AX function leads to genomic instability, radiation sensitivity, and increased cancer susceptibility, while persistent γH2AX accumulation indicates defective DNA repair or replicative stress in cancer cells.

The AlphaLISA SureFire Ultra Mouse Total H2AX Detection Kit is a sandwich immunoassay for the quantitative detection of total H2AX in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies