Revvity AlphaLISA SureFire Ultra Human and Mouse Total NRAS Detection Kit, 50,000 Assay Points

Neuroblastoma RAS Viral Oncogene Homolog (NRAS) is a small GTPase belonging to the RAS family that regulates cell growth, differentiation, and survival through multiple downstream effector pathways. Like other RAS proteins, NRAS cycles between inactive GDP-bound and active GTP-bound conformations, with activation mediated by receptor tyrosine kinases and GEFs. NRAS activates RAF/MEK/ERK, PI3K/AKT, and RAL-GDS pathways to control cellular responses. Activating mutations in NRAS, predominantly at codons 12, 13, and 61, result in impaired GTP hydrolysis and constitutive pathway activation. NRAS mutations are particularly common in melanoma (15-20%), acute myeloid leukemia, and myelodysplastic syndromes, where they drive oncogenesis and confer resistance to certain targeted therapies. NRAS-mutant melanomas show resistance to BRAF inhibitors, necessitating alternative therapeutic approaches including MEK inhibitors.

The AlphaLISA SureFire Ultra Human and Mouse Total NRAS Detection Kit is a sandwich immunoassay for the quantitative detection of total NRAS in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies