Revvity AlphaLISA SureFire Ultra Human and Mouse Total STAT3 High Performance Detection Kit, 50,000 Assay Points

Signal Transducer and Activator of Transcription 3 (STAT3) is a transcription factor that mediates cellular responses to cytokines and growth factors, regulating genes involved in proliferation, survival, and immune responses. STAT3 is activated by phosphorylation at Tyr705, leading to dimerization, nuclear translocation, and DNA binding. STAT3 regulates expression of genes including c-MYC, cyclin D1, BCL-XL, and VEGF, promoting cell cycle progression and survival. Constitutive STAT3 activation is found in approximately 70% of tumors, where it drives oncogenesis and creates an immunosuppressive microenvironment. STAT3 inhibitors are being developed as cancer therapeutics to target tumor cells and restore anti-tumor immunity.

The AlphaLISA SureFire Ultra High Performance Human and Mouse Total STAT3 is a sandwich immunoassay for the quantitative detection of total STAT3 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies