Revvity AlphaLISA SureFire Ultra Mouse Total BAX Detection Kit, 50,000 Assay Points

BCL-2 Associated X-protein (BAX) is a pro-apoptotic member of the BCL-2 family that mediates mitochondrial outer membrane permeabilization to initiate the intrinsic apoptotic pathway. Upon apoptotic stimulation, BH3-only proteins activate BAX, causing translocation to mitochondria, oligomerization, and pore formation. BAX oligomers release cytochrome c and other pro-apoptotic factors that activate caspases and execute cell death. Loss of BAX function contributes to cancer development, chemotherapy resistance, and autoimmune diseases through impaired apoptotic responses. Therapeutic strategies aim to restore BAX-mediated apoptosis through BH3 mimetics or direct BAX activators.

The AlphaLISA SureFire Ultra Mouse Total BAX is a sandwich immunoassay for the quantitative detection of total BAX in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies