Corning 500 mL Dmem (Dulbecco’S Modified Eagle’S Medium)/Hams F-12 50/50 Mix

[+] L-glutamine, 15 mm HEPESThis 1:1 mixture of Dulbeccos Modified Eagle Medium and Hams F-12 media is ideal for culture of a variety of cell types under low serum conditions. Dulbeccos Modified Eagles Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. Hams F-12 media is based on Hams F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids. The formulation contains L-glutamine and 15 mm HEPES. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells.