Corning 500 mL Dmem (Dulbecco’S Modified Eagle’S Medium)

[+] 4.5 g/L glucose, 25 mm HEPES, sodium pyruvate[-] L-glutamine Substitution of Pyridoxine HCL for Pyridoxal ensures improved stability over the original formulation.Dulbeccos Modified Eagles Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of normal and transformed cells, such as primary cultures of mouse and chicken cells. DMEM formulations can differ in the amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine. DMEM 15-018-CV and -CM contain 4.5 g/L glucose, sodium pyruvate, and HEPES at 25 mM, and do not contain L-glutamine.The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells.