Takara Guide-it Genotype Confirmation Kit \100 Rxns

Takara The CRISPR/Cas9 system has been harnessed to create a simple, RNA-programmable method to mediate genome editing in mammalian cells. CRISPR/Cas9 editing generates insertions or deletions (indels) that can result in gene knockout. In most cases, cells have two copies of any given gene and indel mutations can be generated in either one or both alleles. The Guide-it Genotype Confirmation Kit provides a simple protocol to determine whether gene editing resulted in indels on one allele (monoallelic) or both alleles (biallelic) in singly isolated cells (clones), allowing for the identification of clones with desired mutations for further analysis. The kit is designed to be used in conjunction with the Guide-it sgRNA In Vitro Transcription Kit (Cat. No. 632635), which generates a target-specific guide RNA used in the genotype confirmation reaction. The sequence of the guide RNA used for the genotype confirmation reaction is identical to the guide RNA used for the initial genome editing experiment. - TAKBGN (Additional S&H or Hazmat Fees May Apply)