Takara Guide-it CRISPR/Cas9 Gesicle Packaging Set \10 Rxns

Takara Gesicles are cell-derived nanovesicles used to deliver macromolecular cargoes to a broad range of target cells, including cells that are difficult to transfect with plasmids. The nanovesicles generated using the Guide-it CRISPR/Cas9 Gesicle Packaging Set are produced in a Gesicle Producer 293T Cell Line (Cat. No. 632617) via co-overexpression of packaging mix components, which include a nanovesicle-inducing glycoprotein and a protein that is displayed on the cell surface and mediates binding and fusion with the cellular membrane of target cells. Simultaneous overexpression of additional macromolecular cargoes, in this case the Cas9 protein from Streptococcus pyogenes and a target-specific guide RNA (sgRNA), results in incorporation of the Cas9/sgRNA complex within the gesicles. After the resulting Cas9/sgRNA gesicles are harvested and applied to your target cells in the presence of protamine sulfate, they will efficiently enter the cells and mediate gene editing. The Guide-it CRISPR/Cas9 Gesicles Packaging Mixes 1 and 2 are each supplied prealiquoted and lyophilized in single vials containing Xfect Transfection Reagent and an optimized combination of gesicle-producing components. Gesicles are produced by sequentially reconstituting the lyophilized mixtures with your guide RNA vector of choice in sterile water and transfecting the sample into Gesicle Producer 293T cells according to the protocol in the Guide-it CRISPR/Cas9 Gesicle Production System User Manual. It is essential to use the pGuide-itsgRNA1 Vector for expression/packaging of your guide RNA, since using other commonly used guide RNA vectors will not result in effective Cas9/sgRNA complexes. - TAKBGN (Additional S&H or Hazmat Fees May Apply)