Takara Mir-X miRNA First-Strand Synthesis Kit \20 Rxns

This 20 rxn kit contains reagents for synthesizing first-strand cDNA from either total RNA or purified small RNAs isolated from any source. RNA molecules are polyadenylated and reverse transcribed using the mRQ Enzyme Mix, which contains poly(A) polymerase and SMART MMLV RT. The resulting cDNA and Takara Bios TB Green Advantage qPCR Premix can be used in a real-time qPCR analysis to quantify specific miRNAs and other RNAs present in the original sample. For 60 rxn kit see Cat. # 638315.

Mir-X miRNA qRT-PCR TB Green Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green Advantage qPCR chemistry.In the Mir-X cDNA synthesis reaction, RNAs are poly(A)-tailed using poly(A) polymerase, and then copied using a modified oligo(dT) primer and SMART MMLV Reverse Transcriptase.

Specific quantification of Let7 miRNA variants.Using miRNA-specific primers (Panel A), Mir-X qRT-PCR was able to specifically detect and quantify each member of a series of 8 synthetic Let7 variants that had been spiked into a background of yeast poly(A)+RNA (Panel B).The primers detected each of their corresponding Let7 miRNA cognates, but did not detect the off-target variants in 63 of 64 possible combinations.