Takara Library Quantification Kit \500 Rxns

The Library Quantification Kit is used for rapid library quantification prior to Illumina next-generation sequencing. The kit contains all of the components needed to determine the amount of library DNA (genomic, cDNA, etc.) in a sample using qPCR. Quantification is accomplished by comparison of the threshold cycle (Ct) of the diluted sample with a standard curve derived from four calibrated DNA standards.

Lot-to-lot variation for DNA Standards.Quantification of three lots of pre-diluted DNA Standards (0.01, 0.1, 1, and 10 pM) was performed by real-time PCR using Terra qPCR Direct TB Green Premix following the protocol in the Library Quantification Kit user manual. All reactions were performed in triplicate, and the resulting Ct (SDM) values for the triplicates were averaged. The variation between lots was <5%.