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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IP | Rb | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-824 |
| Description | Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal |
| Alternate Names |
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| Background Information | Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics. |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Control |
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| Presentation | Cultured supernatant containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Estrogen Receptor β using this Anti-Estrogen Receptor β Antibody, clone 68-4 validated for use in WB & IP. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: 10 μL of a previous lot immunoprecipitated estrogen receptor β from 500 μg of MCF7 RIPA lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide corresponding to amino acids 63-82 of rat and mouse estrogen receptor β and amino acids 55-74 of human estrogen receptor β. |
| Epitope | a.a.55-74(H) or 63-82 (R, M) |
| Clone | 68-4 |
| Host | Rabbit |
| Specificity | Estrogen receptor β, long form, does not detect estrogen receptor alpha. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Reacts with human. Predicted to cross-react with mouse and rat based on sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq]. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. SUBUNIT: Interacts with SLC30A9 By similarity. Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1. Interaction with MUC1 is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2 and FAM120B. Interacts with isoform 4 of TXNRD1. Interacts with MLL2. Interacts with ATAD2 and this interaction is enhanced by estradiol. SUBCELLULAR LOCATION: Nucleus. DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity. Ref.8 Ref.10 Ref.12 Ref.15 Ref.32 Glycosylated; contains N-acetylglucosamine, probably O-linked. Ref.13 Polymorphism Genetic variations in ESR1 are correlated with bone mineral density (BMD). Low BMD is a risk factor for osteoporotic fracture. Osteoporosis is characterized by reduced bone mineral density, disrutption of bone microarchitecture, and the alteration of the amount and variety of non-collagenous proteins in bone. Osteoporotic bones are more at risk of fracture. SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain. |
| Molecular Weight | 59kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western blot on RIPA lysates from MCF-7 cells. Western Blot Analysis: A 1:500 to 1:2,000 dilution of this antibody detected estrogen receptor β in RIPA lysates from MCF-7 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |